Showing posts with label manhattan plot. Show all posts
Showing posts with label manhattan plot. Show all posts

Tuesday, March 19, 2013

Manhattan plot using Excel

Manhattan plots


Manhattan plots are simply scatter plots where the physical distance are in x axis and p-value or -log10(pvalue) in Y axis. As there are multiple linkage groups, we might want to create a gap and use different color or symbol for different chromosomes.

(1) Data manipulation: We need to create additional column where we put cumulative distances (column dposition) starting from first chromosome, so that we can plot all chromosome in a single scatter plot. If would like to create gap between chromosomes simply add some (may be 10% of average chromosomal length ) value to first value.


(2) Plot dposition (X) vs Y  is -log10(pvalue) using scatter plot function. In addition if would like to use different color or symbol type for different chromosomes manually select each chromosome X and Y cell values. It is much easier to select if the data is in wide format than long format. Now you can remove the all -log10(Pavlue) series. Also you can remove the cumulative positions.


The plotting is similar in other excel version. The idea is basically same. The example output look like the following in 2013, with p-values populated and using different styles for scatter plot.